Reduced transmission of IHHNV to Penaeus monodon from shrimp pond wastewater filtered through a polychaete-assisted sand filter (PASF) system

A polychaete-assisted sand filter (PASF) can aid water recirculation systems for shrimp culture ponds by removing large amounts of nutrients and suspended solids chemically treating wastewater. By ingesting degrading organic matter, Perinereis helleri polychaetes reared in a PASF accumulate potentially remove infectious hypodermal hematopoietic necrosis virus (IHHNV) present wastewater from rearing virus-infected Penaeus monodon shrimp. Reported here are data showing that filtering pond through reduces its potential to transmit IHHNV. The trial employed 3 groups 4 tanks each containing 20 P. randomly selected which only low-level IHHNV infection was evident at moderate prevalence (IHHNV-low Pond 2). Over 2 week period, tank group supplied with either the same (Pond 2) or IHHNV-high 1, 100% prevalent ~10 -fold higher loads, directly after being filtered PASF. real-time qPCR on total nucleic acid (TNA) extracted pleopods 35 random identified an elevated (91%) tested 1. In comparison, detected much reduced lower loads among (33%) 1 it had been (31%). load indicate play useful role reducing naïve . • Filtering this Polychaete-Assisted Sand Filter drastically transmission. prevalence/loads shown increase exposed acute infection. Prevalence 2/3 when were alternatively following treatment Results suggest be